PE anti-mouse I-A-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1464	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C57BL/10J splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed⁵; OCT-embedded, ethanol-fixed sections⁷), immunofluorescence microscopy³ (including acetone-fixed epidermal sheets⁶), immunoprecipitation^7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Does not react with other haplotypes (e.g., d, f, q, r, s).

Application References (PubMed link indicates BioLegend citation)

Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.

Product Citations

Beuneu H, et al. 2006. J Immunol . 177:1406. PubMed
Wu J, et al. 2021. Mol Med Rep. 23:. PubMed
Liang Y, et al. 2014. J Immunol. 192:1277. PubMed
Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
Zager A, et al. 2017. Progress in Neuro-Psychopharmacology and Biological Psychiatry. 10.1016/j.pnpbp.2017.05.003. PubMed
Song S, et al. 2021. Front Immunol. 12:705140. PubMed
Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed

RRID

AB_313726 (BioLegend Cat. No. 116407) AB_313727 (BioLegend Cat. No. 116408)

Antigen Details

Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2^b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
Gene ID: 14961 View all products for this Gene ID
UniProt: View information about I-Ab on UniProt.org

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