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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse I-A
产品名称：
Pacific Blue™ anti-mouse I-A
产品类别：
抗体
产品编号：
116421
产品应用：
116421
[价格]
规格 价格 库存
25µg ￥ 1272 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10J splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Does not react with other haplotypes (e.g., d, f, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
  2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
  3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
  4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
  5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
  6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
  7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
  8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
  9. Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
Product Citations
  1. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  2. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  3. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
  4. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  5. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  6. Tay S, et al. 2014. J Immunol. 193:2087. PubMed
  7. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  8. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  9. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  10. Ruscher R, et al. 2018. Bio Protoc. 8:e2757. PubMed
  11. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  12. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
RRID
AB_10613291 (BioLegend Cat. No. 116421) AB_10613473 (BioLegend Cat. No. 116422)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ab on UniProt.org

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