Brilliant Violet 421™ anti-human Fcε-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2448	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.⁴

Application References (PubMed link indicates BioLegend citation)

Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
Suzukawa M, et al. 2005. Int. Immunol. 17:1249.
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Yamaguchi M, et al. 1999. J. Immunol. 162:5455.

Product Citations

Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
Dyring-Andersen B, et al. 2020. Nat Commun. 4.338194444. PubMed
Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed

RRID

AB_2564289 (BioLegend Cat. No. 334623) AB_2564290 (BioLegend Cat. No. 334624)

Antigen Details

Structure: Ig superfamily, 50 kD
Distribution: Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients
Function: Bind IgE, trigger IgE-mediated allergic response
Ligand/Receptor: IgE
Cell Type: Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils
Biology Area: Immunology
Molecular Family: Fc Receptors
Antigen References: 1. Riske F, et al. 1991. J. Biol. Chem. 266:11245
2. Gounni AS, et al. 2001. FASEB J. 15:940.
3. Maurer D, et al. 1996. J. Immunol. 157:607
4. Maurer d, et al. 1994. J. Exp. Med. 179:745
5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92.
Gene ID: 2205 View all products for this Gene ID
UniProt: View information about FcepsilonRIalpha on UniProt.org

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