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Purified anti-human CD73 (Ecto-5'-nucleotidas
产品名称:
Purified anti-human CD73 (Ecto-5'-nucleotidas
产品类别:
抗体
产品编号:
344002
产品应用:
344002
[价格]
规格 价格 库存
100µg ¥ 2220 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.?For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  4. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  5. Garita-Hernández M, et al. 2021. Front Cell Neurosci. 15:648210. PubMed
  6. Xue G, et al. 2021. Nat Biomed Eng. 5:1306. PubMed
  7. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  8. Messaoudi N, et al. 2020. Oncoimmunology. 9:1746138. PubMed
  9. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  10. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_2154067 (BioLegend Cat. No. 344002)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen? S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

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