Brilliant Violet 421™ anti-human CD73 (Ecto-
- 产品名称：
- Brilliant Violet 421™ anti-human CD73 (Ecto-
- 产品类别：
- 抗体
- 产品编号：
- 344007
- 产品应用：
- 344007
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2280 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include:immunofluorescence3.
Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.
- Application References
(PubMed link indicates BioLegend citation) -
- Nakamura T, et al. 1993. J. Immunol. 151:6933.
- Liao J, et al. 2011. J Endod. 37:1217. PubMed
- Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
- Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
- Product Citations
-
- Kaneko N, et al. 2020. Cell. 183(1):143-157.e13. PubMed
- Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
- Ng E, et al. 2016. Nat Biotechnol. 34:1168-1179. PubMed
- Harvestine JN, et al. 2018. J Mater Chem B. 6:4104. PubMed
- Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
- Harvestine JN, et al. 2020. Sci Adv. 6:eaay2387. PubMed
- Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
- Yang YK, et al. 2021. Stem Cell Res Ther. 12:156. PubMed
- RRID
- AB_10900981 (BioLegend Cat. No. 344007) AB_11204424 (BioLegend Cat. No. 344008)
- Structure
- GPI-linked 5'-nucleotidase, 70 kD
- Distribution
-
Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
- Function
- Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen? S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.
- Gene ID
- 4907 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
