PE anti-mouse CD285 (TLR5) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2124	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

TLR5-transfected Ba/F3 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates^1,2.

Application References (PubMed link indicates BioLegend citation)

Shibata T, et al. Int Immunol. 24:613. (IP, FC)
Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)

RRID

AB_2890696 (BioLegend Cat. No. 148107) AB_2890696 (BioLegend Cat. No. 148108)

Antigen Details

Structure: Type 1 transmembrane protein, 98 kD
Distribution: Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium
Interaction: Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor: Flagellin
Cell Type: Dendritic cells, Monocytes, Neutrophils
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587.?
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857.?
3. Carapau D, et al. 2013. Infect. Immun. 81:4350.?
4. Kinnebrew M, et al. 2012. Immunity. 36:276.?
5. Sebastiani G, et al. 2000. Genomics. 64:230.
Gene ID: 53791 View all products for this Gene ID
UniProt: View information about CD285 on UniProt.org

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