Alexa Fluor® 647 anti-mouse CD285 (TLR5) Antib
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD285 (TLR5) Antib
- 产品类别：
- 抗体
- 产品编号：
- 148103
- 产品应用：
- 148103
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- TLR5-transfected Ba/F3 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.
- Application References
(PubMed link indicates BioLegend citation) -
- Shibata T, et al. Int Immunol. 24:613. (IP, FC)
- Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)
- Product Citations
-
- Xu Y, et al. 2021. iScience. 24:103445. PubMed
- RRID
- AB_2563694 (BioLegend Cat. No. 148103) AB_2563695 (BioLegend Cat. No. 148104)
- Structure
- Type 1 transmembrane protein, 98 kD
- Distribution
-
Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium
- Interaction
- Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
- Ligand/Receptor
- Flagellin
- Cell Type
- Dendritic cells, Monocytes, Neutrophils
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587.?
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857.?
3. Carapau D, et al. 2013. Infect. Immun. 81:4350.?
4. Kinnebrew M, et al. 2012. Immunity. 36:276.?
5. Sebastiani G, et al. 2000. Genomics. 64:230.
- Gene ID
- 53791 View all products for this Gene ID
- UniProt
- View information about CD285 on UniProt.org
