Ultra-LEAF™ Purified anti-mouse CD117 (c-kit-上海雅吉生物科技有限公司

规格	价格	库存
1mg	￥ 6936	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Murine IL-3 dependent mast cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.³

ACK2 has been reported to block c-Kit function. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Yoshinaga K,?et al.?1991.?Development?113:689.
Broudy VC,?et al.?1996.?Blood?88:75.
Louvet C,?et al.?2008.?Proc. Natl. Acad. Sci. USA?105:18895.

Product Citations

Barkaway A, et al. 2021. Immunity. . PubMed
Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed

RRID

AB_2561477 (BioLegend Cat. No. 135114) AB_2571992 (BioLegend Cat. No. 135131) AB_2571993 (BioLegend Cat. No. 135132)

Antigen Details

Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Stem Cell Factor (SCF)
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.
Gene ID: 16590 View all products for this Gene ID
UniProt: View information about CD117 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们