PE anti-mouse CD117 (c-kit) Antibody
- 产品名称：
- PE anti-mouse CD117 (c-kit) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 135105
- 产品应用：
- 135105
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine IL-3 dependent mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3
ACK2 has been reported to block c-Kit function. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Application References
(PubMed link indicates BioLegend citation) -
- Yoshinaga K,?et al.?1991.?Development?113:689.
- Broudy VC,?et al.?1996.?Blood?88:75.
- Louvet C,?et al.?2008.?Proc. Natl. Acad. Sci. USA?105:18895.
- Product Citations
-
- Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
- Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
- Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
- RRID
- AB_1877216 (BioLegend Cat. No. 135105) AB_1877215 (BioLegend Cat. No. 135106)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
