Brilliant Violet 605™ anti-mouse CD117 (c-ki
- 产品名称：
- Brilliant Violet 605™ anti-mouse CD117 (c-ki
- 产品类别：
- 抗体
- 产品编号：
- 135121
- 产品应用：
- 135121
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine IL-3 dependent mast cells
- Formulation
- ?l sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mL?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3
ACK2 has been reported to block c-Kit function. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Application References
(PubMed link indicates BioLegend citation) -
- Yoshinaga K,?et al.?1991.?Development?113:689.
- Broudy VC,?et al.?1996.?Blood?88:75.
- Louvet C,?et al.?2008.?Proc. Natl. Acad. Sci. USA?105:18895.
- Product Citations
-
- Oetjen LK et al. 2017. Cell. 171(1):217-228 . PubMed
- Chen S, et al. 2022. Cancer Discov. :. PubMed
- Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
- Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
- Yoshimi A, et al. 2019. Nature. 574:273. PubMed
- Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
- Green DP, et al. 2019. Neuron. 101:412. PubMed
- Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
- Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
- Gentek R, et al. 2018. Immunity. 48:1160. PubMed
- Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
- RRID
- AB_2562040 (BioLegend Cat. No. 135121) AB_2650925 (BioLegend Cat. No. 135120) AB_2562042 (BioLegend Cat. No. 135122)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
