APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody
- 产品名称：
- APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 135135
- 产品应用：
- 135135
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine IL-3 dependent mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3
ACK2 has been reported to block c-Kit function. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Yoshinaga K,?et al.?1991.?Development?113:689.
- Broudy VC,?et al.?1996.?Blood?88:75.
- Louvet C,?et al.?2008.?Proc. Natl. Acad. Sci. USA?105:18895.
- Product Citations
-
- Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
- Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
- Zhang B, et al. 2020. Nature. 577:676. PubMed
- Luo H, et al. 2020. Cancer Cell. 36(6):645-659.e8.. PubMed
- Luo H, et al. 2022. Mol Cell. 82:833. PubMed
- Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
- Hacein-Bey-Abina S, et al. 2020. Exp Hematol. 88:15. PubMed
- RRID
- AB_2632808 (BioLegend Cat. No. 135135) AB_2632809 (BioLegend Cat. No. 135136)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
