Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3) A-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2112	1

Product Details

Verified Reactivity

Mouse, Rat, Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 ?g per million cells in 100 ?L?volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 1.25-5 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Application References (PubMed link indicates BioLegend citation)

Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Lin YC, et al. 2021. Nat Neurosci. 24:1077. PubMed
Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
Buljan M, et al. 2020. Molecular Cell. 79(3):504-520. PubMed

RRID

AB_2563609 (BioLegend Cat. No. 657405) AB_2563610 (BioLegend Cat. No. 657406)

Antigen Details

Structure: 450 amino acids with predicted molecular weight of 50 kD
Distribution: Cytosol
Function: Plays important roles in neuronal cell proliferation and differentiation
Interaction: Alpha tubulin, kinesin and dynein
Cell Type: Mature Neurons
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Antigen References: 1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.
Gene ID: 10381 View all products for this Gene ID
UniProt: View information about Tubulin beta-3 on UniProt.org

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