Ultra-LEAF™ Purified anti-mouse CD127 (IL-7R-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

IL-7Ra-IgG1 fusion protein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, WB, Block, IHC, ICC?- Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Application References (PubMed link indicates BioLegend citation)

Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
Hashi H, et al. 2001. J. Immunol. 166:3702.
Taylor R, et al. 2007. J. Immunol. 178:5659.
Mazzon C, et al. 2011. Blood. 118:2733. PubMed
Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed

RRID

AB_2832473 (BioLegend Cat. No. 135049) AB_2832474 (BioLegend Cat. No. 135050) AB_2832475 (BioLegend Cat. No. 135051) AB_2832476 (BioLegend Cat. No. 135052) AB_2832477 (BioLegend Cat. No. 135053) AB_2832478 (BioLegend Cat. No. 135054)

Antigen Details

Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
Gene ID: 16197 View all products for this Gene ID
UniProt: View information about CD127 on UniProt.org

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