PE/Cyanine5 anti-mouse CD127 (IL-7Rα) Antibo
- 产品名称：
- PE/Cyanine5 anti-mouse CD127 (IL-7Rα) Antibo
- 产品类别：
- 抗体
- 产品编号：
- 135015
- 产品应用：
- 135015
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-7Ra-IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.15 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
A7R34 is able to block clone SB/199 binding to IL-7R.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
- Hashi H, et al. 2001. J. Immunol. 166:3702.
- Taylor R, et al. 2007. J. Immunol. 178:5659.
- Mazzon C, et al. 2011. Blood. 118:2733. PubMed
- Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
- Product Citations
-
- Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
- Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
- Peng X, et al. 2014. J Immunol. 193:1268. PubMed
- Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
- Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
- Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
- Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
- Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
- Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
- Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
- Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
- Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
- RRID
- AB_1937262 (BioLegend Cat. No. 135015) AB_1937261 (BioLegend Cat. No. 135016)
- Structure
- Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
- Distribution
-
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
- Function
- T cell and immature B cell proliferation and development
- Ligand/Receptor
- IL-7
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
- Gene ID
- 16197 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org
