Brilliant Violet 605™ anti-mouse CD127 (IL-7
- 产品名称：
- Brilliant Violet 605™ anti-mouse CD127 (IL-7
- 产品类别：
- 抗体
- 产品编号：
- 135025
- 产品应用：
- 135025
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-7Ra-IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is 0.6 ?g per million cells in 100 ?l volume. For flow cytometric staining using the test size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
A7R34 is able to block clone SB/199 binding to IL-7R.
- Application References
(PubMed link indicates BioLegend citation) -
- Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
- Hashi H, et al. 2001. J. Immunol. 166:3702.
- Taylor R, et al. 2007. J. Immunol. 178:5659.
- Mazzon C, et al. 2011. Blood. 118:2733. PubMed
- Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
- Product Citations
-
- Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
- Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
- Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
- Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
- Progatzky F, et al. 2021. Nature. 599:125. PubMed
- Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
- Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed
- Lee W, et al. 2021. J Virol. 95:e0053021. PubMed
- Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed
- Zeis P, et al. 2020. Immunity. 53:775. PubMed
- Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
- Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
- Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed
- Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
- Dingler FA, et al. 2020. Mol Cell. . PubMed
- RRID
- AB_2562114 (BioLegend Cat. No. 135025) AB_2572047 (BioLegend Cat. No. 135041)
- Structure
- Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
- Distribution
-
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
- Function
- T cell and immature B cell proliferation and development
- Ligand/Receptor
- IL-7
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
- Gene ID
- 16197 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org
