Alexa Fluor® 647 anti-mouse CD127 (IL-7R&alpha
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD127 (IL-7R&alpha
- 产品类别：
- 抗体
- 产品编号：
- 135019
- 产品应用：
- 135019
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-7Ra-IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
A7R34 is able to block clone SB/199 binding to IL-7R.
- Application References
(PubMed link indicates BioLegend citation) -
- Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
- Hashi H, et al. 2001. J. Immunol. 166:3702.
- Taylor R, et al. 2007. J. Immunol. 178:5659.
- Mazzon C, et al. 2011. Blood. 118:2733. PubMed
- Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
- Product Citations
-
- Doisne J, et al. 2015. J Immunol. 195: 3937 - 3945.. PubMed
- Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
- Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
- Shen B, et al. 2021. Nature. 591:438. PubMed
- Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
- RRID
- AB_1937210 (BioLegend Cat. No. 135019) AB_1937209 (BioLegend Cat. No. 135020)
- Structure
- Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
- Distribution
-
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
- Function
- T cell and immature B cell proliferation and development
- Ligand/Receptor
- IL-7
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
- Gene ID
- 16197 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org
