| 规格 | 价格 | 库存 |
|---|---|---|
| 50µg | ¥ 1632 | 1 |
ELISA Capture - Quality tested
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of human CTLA-4 recombinant protein ranging from 2500 to 39 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
ELISA Capture: The purified A3.6B10.G1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated L3D10 antibody (Cat. No. 349911) as the detection antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.
Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX? Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407 & 437408) are specially developed and recommended.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).
Activated T cells and B cells
1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.