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Purified anti-human CD152 (CTLA-4) Antibody
产品名称:
Purified anti-human CD152 (CTLA-4) Antibody
产品类别:
抗体
产品编号:
525401
产品应用:
525401
[价格]
规格 价格 库存
50µg ¥ 1632 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of human CTLA-4 recombinant protein ranging from 2500 to 39 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified A3.6B10.G1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated L3D10 antibody (Cat. No. 349911) as the detection antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.

Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX? Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407 & 437408) are specially developed and recommended.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).

RRID
AB_2562988 (BioLegend Cat. No. 525401) AB_2563003 (BioLegend Cat. No. 525402)

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T cells and B cells

Function
Negative regulator of T cell activation
Interaction
Antigen presenting cells, such as dendritic cells
Ligand/Receptor
B7-1 (CD80), B7-2 (CD86)
Cell Type
T cells, B cells, Dendritic cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.

Gene ID
1493 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

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