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Brilliant Violet 421™ anti-mouse CD135 Antib
产品名称：
Brilliant Violet 421™ anti-mouse CD135 Antib
产品类别：
抗体
产品编号：
135313
产品应用：
135313
[价格]
规格 价格 库存
125µL ￥ 2184 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g size: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of the this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  2. Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed
  3. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  4. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  5. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  6. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  7. Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
  8. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  9. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  10. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  11. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  12. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  13. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  14. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  15. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  16. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  17. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_2562338 (BioLegend Cat. No. 135313) AB_2571919 (BioLegend Cat. No. 135315) AB_2562339 (BioLegend Cat. No. 135314)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org

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