Ultra-LEAF™ Purified anti-human CD41/CD61 An
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD41/CD61 An
- 产品类别：
- 抗体
- 产品编号：
- 359804
- 产品应用：
- 359804
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human platelets
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 359804).
A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.
- Application References
(PubMed link indicates BioLegend citation) -
- Basani RB, et al. 1996. Blood. 88:167. (IP)
- Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
- Product Citations
-
- Zheng L, et al. 2021. Front Cell Infect Microbiol. 11:783085. PubMed
- Posner M, et al. 2016. J Biol Chem. 291: 2764 - 2776.. PubMed
- RRID
- AB_2562484 (BioLegend Cat. No. 359804)
- Structure
- Complex of CD41 and CD61 through non-covalent association.
- Distribution
- Platelets and megakaryocytes.
- Function
- Mediates attachment to cells of diverse matrix proteins.
- Ligand/Receptor
- Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
- Cell Type
- Platelets, Megakaryocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862.
2. Corbel C, et al.?2005. 49:279.
3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417.?
4. Clemetson KJ, et al.?1994. Curr. Opin. Hematol. 1:388.
- Gene ID
- 3690 View all products for this Gene ID 3674 View all products for this Gene ID
- UniProt
- View information about CD41/CD61 on UniProt.org
