Purified anti-mouse CD45.1 Antibody
- 产品名称：
- Purified anti-mouse CD45.1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 110702
- 产品应用：
- 110702
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SJL mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, Block, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).
- Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
- Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
- Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
- Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
- Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
- Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
- Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
- Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
- Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
- Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
- Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
- Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
- Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
- Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
- Kenna TJ, et al. 2008. Blood 111:2091. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
- Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
- Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed
- Hosoi A, et al. 2008. Cancer Res. 68:3941. PubMed
- Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
- Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
- De Simone G, et al. 2021. Immunity. :. PubMed
- Guilliams M, et al. 2022. Cell. 185:379. PubMed
- Kenna T, et al. 2008. Blood. 111:2091. PubMed
- Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
- Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
- Speir M, et al. 2020. Nat Immunol. 21:54. PubMed
- Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed
- Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
- Basilico S, et al. 2020. Nat Commun. 11:1407. PubMed
- Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
- Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
- Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed
- Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
- RRID
- AB_313491 (BioLegend Cat. No. 110702)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.1 on UniProt.org
