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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse CD45.1 Antibody
产品名称：
PerCP/Cyanine5.5 anti-mouse CD45.1 Antibody
产品类别：
抗体
产品编号：
110727
产品应用：
110727
[价格]
规格 价格 库存
25µg ￥ 1416 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  3. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  4. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  5. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  6. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  7. Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
  8. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  9. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  10. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  11. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  12. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  13. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  14. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  15. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  16. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  17. Marangoni F, et al. 2021. Cell. . PubMed
  18. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  19. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  20. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  21. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  22. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  23. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  24. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  25. Quah B, et al. 2012. Cytometry A. 81:679. PubMed
  26. Sharma S, et al. 2021. Pathophysiology. 28:501. PubMed
  27. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  28. Karimi M, et al. 2015. Blood. 125:3655. PubMed
  29. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  30. Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed
  31. Krueger CC, et al. 2019. Front Immunol. 10:1831. PubMed
  32. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  33. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  34. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  35. LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed
  36. Ma K, et al. 2022. iScience. 25:104347. PubMed
  37. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  38. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  39. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  40. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  41. Lamb T, et al. 2022. Toxics. 10:. PubMed
  42. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  43. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  44. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  45. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  46. X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
  47. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  48. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  49. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  50. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  51. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  52. Ehrens A, et al. 2021. Cell Reports. 34(2):108621. PubMed
RRID
AB_893348 (BioLegend Cat. No. 110727) AB_893346 (BioLegend Cat. No. 110728)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

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