PerCP anti-mouse CD45.1 Antibody
- 产品名称：
- PerCP anti-mouse CD45.1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 110726
- 产品应用：
- 110726
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SJL mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).
- Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
- Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
- Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
- Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
- Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
- Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
- Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
- Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
- Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
- Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
- Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
- Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
- Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
- Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
- Kenna TJ, et al. 2008. Blood 111:2091. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
- Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
- Canto F, et al. 2016. Sci Rep. 6:28573. PubMed
- Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
- Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
- Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
- Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
- Nijnik A, et al. 2012. J Immunol . 189:102. PubMed
- Skokowa J, et al. 2022. Nat Commun. 13:2948. PubMed
- Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
- Koide S, et al. 2022. iScience. 25:103603. PubMed
- Sheng J, et al. 2021. eLife. 10:00. PubMed
- Luo H, et al. 2019. Cell Rep. 26:945. PubMed
- Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
- Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
- RRID
- AB_893347 (BioLegend Cat. No. 110725) AB_893345 (BioLegend Cat. No. 110726)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.1 on UniProt.org
