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首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-mouse CD45.1 Antibody
产品名称：
PE/Cyanine7 anti-mouse CD45.1 Antibody
产品类别：
抗体
产品编号：
110729
产品应用：
110729
[价格]
规格 价格 库存
25µg ￥ 792 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
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  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
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  2. Burghardt S, et al. 2013. J Immunol. 191:5574. PubMed
  3. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
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  12. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  13. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  14. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  15. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  16. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  17. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  18. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  19. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  20. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  21. Geng S, et al. 2021. JCI Insight. 6:. PubMed
  22. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  23. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  24. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  25. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  26. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  27. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  28. Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed
  29. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  30. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  31. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  32. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  33. Subedi A, et al. 2021. Cell Stem Cell. :. PubMed
  34. Lebel M?, et al. 2020. Nat Commun. 3.051388889. PubMed
  35. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  36. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  37. Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed
  38. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  39. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  40. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  41. Koide S, et al. 2022. iScience. 25:103603. PubMed
  42. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  43. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  44. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
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  47. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  48. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  49. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
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  51. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  52. Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed
  53. Barrigan L, et al. 2013. Infect Immun . 81:2028. PubMed
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RRID
AB_1134170 (BioLegend Cat. No. 110729) AB_1134168 (BioLegend Cat. No. 110730)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

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