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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse CD45.1 Antibody
产品名称：
Pacific Blue™ anti-mouse CD45.1 Antibody
产品类别：
抗体
产品编号：
110721
产品应用：
110721
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  3. Page N, et al. 2018. Immunity. 48:937. PubMed
  4. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  5. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  6. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  7. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  8. Pack AD, et al. 2021. Cell Reports. 36(8):109586. PubMed
  9. Lin G, et al. 2013. J Immunol. 190:4627. PubMed
  10. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  11. Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
  12. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  13. Müller M, et al. 2021. Nat Commun. 12:7036. PubMed
  14. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  15. Li L, et al. 2010. J Immunol. 184:1728. PubMed
  16. Wigton E, et al. 2016. J Leukoc Biol. 100: 143 - 153. PubMed
  17. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  18. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  19. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  20. Cheng Y, et al. 2021. Cancer Cell. . PubMed
  21. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  22. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  23. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  24. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  25. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  26. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  27. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  28. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  29. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  30. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  31. Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed
  32. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  33. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  34. Holian C 2007. Am J Respir Cell Mol Biol. 37:729. PubMed
  35. Florez MA, et al. 2020. Cell Rep. 33:108530. PubMed
  36. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
  37. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  38. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  39. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  40. Mills R, et al. 2015. J Immunol. 194:3675. PubMed
  41. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  42. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  43. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
  44. Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed
  45. Fiege JK, et al. 2019. J Immunol. 203(4):936-945. PubMed
  46. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  47. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  48. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  49. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  50. Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed
  51. Yá?ez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_492867 (BioLegend Cat. No. 110721) AB_492866 (BioLegend Cat. No. 110722)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

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