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Brilliant Violet 421™ anti-mouse CD45.1 Anti
产品名称：
Brilliant Violet 421™ anti-mouse CD45.1 Anti
产品类别：
抗体
产品编号：
110731
产品应用：
110731
[价格]
规格 价格 库存
125µL ￥ 2184 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. For immunofluorescent staining using the ?L size, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Xu K, et al. 2021. Immunity. 54(5):976-987.e7. PubMed
  3. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  4. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  5. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  6. Marangoni F, et al. 2021. Cell. . PubMed
  7. Ning F, et al. 2019. J Exp Med. 216:1396. PubMed
  8. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  9. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  10. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  11. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  12. Zhang X, et al. 2020. Elife. 9:00. PubMed
  13. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  14. Xu S, et al. 2021. Immunity. . PubMed
  15. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  16. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  17. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  18. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  19. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  20. Danzer H, et al. 2020. Elife. 9:00. PubMed
  21. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  22. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  23. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  24. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  25. Goldberg MF et al. 2018. Immunity. 49(6):1090-1102 . PubMed
  26. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
  27. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
RRID
AB_10896425 (BioLegend Cat. No. 110731) AB_2562563 (BioLegend Cat. No. 110732)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

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