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首页 > 抗体蛋白 > 抗体
APC/Cyanine7 anti-mouse CD45.1 Antibody
产品名称：
APC/Cyanine7 anti-mouse CD45.1 Antibody
产品类别：
抗体
产品编号：
110715
产品应用：
110715
[价格]
规格 价格 库存
25µg ￥ 1320 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed
  2. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  3. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  4. Le Q, et al. 2021. Clin Cancer Res. Online ahead of prin. PubMed
  5. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  6. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  7. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  8. Frame JM, et al. 2020. Developmental Cell. 55(2):133-149.e6. PubMed
  9. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
  10. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  11. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  12. Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed
  13. Amor C, et al. 2020. Nature. 583:127. PubMed
  14. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  15. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  16. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  17. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  18. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  19. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  20. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  21. Li J, et al. 2018. Immunity. 49:178. PubMed
  22. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  23. Sun L, et al. 2021. Cell Reports. 35(9):109188. PubMed
  24. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  25. De Simone G, et al. 2021. Immunity. :. PubMed
  26. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  27. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  28. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  29. Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed
  30. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  31. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  32. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  33. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  34. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  35. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  36. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  37. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  38. Wang X, et al. 2022. Elife. 11:. PubMed
  39. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  40. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  41. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_313504 (BioLegend Cat. No. 110715) AB_313505 (BioLegend Cat. No. 110716)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

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