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Alexa Fluor® 700 anti-mouse CD45.1 Antibody
产品名称：
Alexa Fluor® 700 anti-mouse CD45.1 Antibody
产品类别：
抗体
产品编号：
110723
产品应用：
110723
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or?mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
  3. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  4. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  5. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  6. Withers D, et al. 2009. J Immunol. 183:5079. PubMed
  7. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  8. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  9. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  10. Garidou L, et al. 2012. J Virol. 86:7060. PubMed
  11. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  12. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  13. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  14. Huang W, et al. 2014. J Immunol. 193:2267. PubMed
  15. Semmrich M, et al. 2011. Mucosal Immunol. 0.3125. PubMed
  16. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  17. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  18. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  19. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  20. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  21. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  22. Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed
  23. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  24. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  25. Baasch S, et al. 2021. Cell. . PubMed
  26. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  27. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  28. Tondini E, et al. 2022. NPJ Vaccines. 7:64. PubMed
  29. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  30. Zhang J, et al. 2021. Nature. 590:457. PubMed
  31. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  32. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  33. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  34. Li C, et al. 2018. Cell. 174:285. PubMed
  35. Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed
  36. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  37. Huang W, et al. 2014. J Leukoc Biol. 96:55. PubMed
  38. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  39. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  40. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  41. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
  42. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  43. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  44. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  45. S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
  46. Eftychi C et al. 2019. Immunity. 51(2):367-380 . PubMed
  47. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  48. Schuran FA, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
  49. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
RRID
AB_493732 (BioLegend Cat. No. 110723) AB_493733 (BioLegend Cat. No. 110724)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

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