Ultra-LEAF™ Purified anti-human CD150 (SLAM)-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Activated human PBMC

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation⁴, and costimulation^1,5 of IFN-gamma production and T cell proliferation. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 306317 and 306318).

Application References (PubMed link indicates BioLegend citation)

Garcia V, et al. 2001. J. Immunol. 167:5719. (Costim)
Vincent S, et al. 2002. J. Virol. 76:6121.
Cocks B, et al. 1995. Nature 376:260.
Sayos J, et al. 2001. Blood 97:3867. (IP)
Aversa G, et al. 1997. J. Immunol. 158:4036. (Costim)
Spencer M, et al. 2010. Am. J. Physiol Endocrinol Metab. 299:1016. PubMed

RRID

AB_2860817 (BioLegend Cat. No. 306317) AB_2860818 (BioLegend Cat. No. 306318)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 70-95 kD
Distribution: T subset (upregulated after activation), B cells, dendritic cells, endothelial cells
Function: B cell costimulation, proliferation and Ig production
Ligand/Receptor: CD150 (self ligand)
Cell Type: B cells, Dendritic cells, Endothelial cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Cocks B, et al. 1995. Nature 376:260.
2. Pinchouk V, et al. 1988. AntiCancer Res. 8:1377.
3. Polacino P, et al. 1996. J. Med. Primatol. 25:201.
4. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
Gene ID: 6504 View all products for this Gene ID
UniProt: View information about CD150 on UniProt.org

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