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首页 > 抗体蛋白 > 抗体
Purified anti-human CD39 Antibody
产品名称：
Purified anti-human CD39 Antibody
产品类别：
抗体
产品编号：
328202
产品应用：
328202
[价格]
规格 价格 库存
100µg ￥ 1344 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA activated human lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade3,? immunofluorescence4,?immunohistochemistry6, and spatial biology (IBEX)7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for blocking assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Aversa GG, et al. 1988. Transplant. P. 20:4952.
  2. Aversa GG, et al. 1989. Transplant. P. 21:34950.
  3. Borsellino G, et al. 2007. Blood. 110:1225. (Block)
  4. Stockl J, et al. 2001. J. Immunol. 167:2724. (IF)
  5. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  6. Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC)
  7. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  8. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  3. Brinkmann CR, et al. 2018. mSphere. 3:e00616. PubMed
  4. Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
  5. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  6. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  7. Olin A, et al. 2018. Cell. 174:1277. PubMed
  8. Henrick BM, et al. 2021. Cell. . PubMed
  9. NULL, et al. 2022. Cell. 185:916. PubMed
  10. Schwabenland M, et al. 2021. Immunity. . PubMed
  11. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  12. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  13. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  14. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
RRID
AB_940438 (BioLegend Cat. No. 328202)

Antigen Details

Distribution

Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.

Cell Type
B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
953 View all products for this Gene ID
UniProt
View information about CD39 on UniProt.org

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