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Purified anti-human CD39 (Maxpar® Ready) Antib
产品名称:
Purified anti-human CD39 (Maxpar® Ready) Antib
产品类别:
抗体
产品编号:
328221
产品应用:
328221
[价格]
规格 价格 库存
100µg ¥ 1692 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA activated human lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF?,?IHC-P - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade3,? immunofluorescence4,?immunohistochemistry6, and spatial biology (IBEX)7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for blocking assays (contact our custom solutions team).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Aversa GG, et al. 1988. Transplant. P. 20:4952.
  2. Aversa GG, et al. 1989. Transplant. P. 21:34950.
  3. Borsellino G, et al. 2007. Blood. 110:1225. (Block)
  4. Stockl J, et al. 2001. J. Immunol. 167:2724. (IF)
  5. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  6. Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC)
  7. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  8. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  2. Gruber CN, et al. 2020. Cell. 183:982. PubMed
RRID
AB_2563747 (BioLegend Cat. No. 328221)

Antigen Details

Distribution

Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.

Cell Type
B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
953 View all products for this Gene ID
UniProt
View information about CD39 on UniProt.org

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