PE/Dazzle™ 594 anti-human CD39 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1548	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

PHA activated human lymphocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade³,? immunofluorescence⁴,?immunohistochemistry⁶, and spatial biology (IBEX)^7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for blocking assays (contact our custom solutions team).

Application References (PubMed link indicates BioLegend citation)

Aversa GG, et al. 1988. Transplant. P. 20:4952.
Aversa GG, et al. 1989. Transplant. P. 21:34950.
Borsellino G, et al. 2007. Blood. 110:1225. (Block)
Stockl J, et al. 2001. J. Immunol. 167:2724. (IF)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
Colleen S McGary et al. 2017. Immunity. 47(4):776-788 . PubMed
Abd Hamid M, et al. 2020. Cancer Immunology Research. 8(2):203-216. PubMed
Vanoni G, et al. 2021. eLife. 10:00. PubMed

RRID

AB_2564318 (BioLegend Cat. No. 328223) AB_2564319 (BioLegend Cat. No. 328224)

Antigen Details

Distribution: Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.
Cell Type: B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 953 View all products for this Gene ID
UniProt: View information about CD39 on UniProt.org

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