Brilliant Violet 421™ anti-human CD39 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2280	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

PHA activated human lymphocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade³,? immunofluorescence⁴,?immunohistochemistry⁶, and spatial biology (IBEX)^7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for blocking assays (contact our custom solutions team).

Application References (PubMed link indicates BioLegend citation)

Aversa GG, et al. 1988. Transplant. P. 20:4952.
Aversa GG, et al. 1989. Transplant. P. 21:34950.
Borsellino G, et al. 2007. Blood. 110:1225. (Block)
Stockl J, et al. 2001. J. Immunol. 167:2724. (IF)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

R?hl M, et al. 2021. Vaccines (Basel). 9:. PubMed
Montané E, et al. 2017. PLoS One. 12(2):e0171294. PubMed
Kim DH, et al. 2021. eLife. 10:00. PubMed
Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
Zou F, et al. 2021. Mol Ther. 29:1794. PubMed
Lam AJ, et al. 2021. Cell Reports. 36(5):109494. PubMed
Ellis-Connell AL, et al. 2018. J Virol. 92:e01748. PubMed
Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed

RRID

AB_10933084 (BioLegend Cat. No. 328213) AB_2564575 (BioLegend Cat. No. 328214)

Antigen Details

Distribution: Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.
Cell Type: B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 953 View all products for this Gene ID
UniProt: View information about CD39 on UniProt.org

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