PerCP/Cyanine5.5 anti-human Galectin-9 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2256	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant peptide from C-terminus of Galectin-9

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested
FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: cell surface staining for flow cytometry¹ and blocking of TIM-3 binding to galectin-9¹.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Klibi J, et al. 2009. Blood 113:1957. (FC, Block)
Sada-Ovalle I, et al. 2012. J. Immunol. 189:5896. PubMed

Product Citations

Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
Petersen SH, et al. 2021. Sci Rep. 11:12264. PubMed

RRID

AB_2562687 (BioLegend Cat. No. 348909) AB_2562688 (BioLegend Cat. No. 348910)

Antigen Details

Structure: Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells.
Function: Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells.
Ligand/Receptor: HAVCR2/TIM3
Cell Type: Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Seki M, et al. 2008. Clin. Immunol. 127:78.
2. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
3. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
4. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
Gene ID: 3965 View all products for this Gene ID
UniProt: View information about Galectin-9 on UniProt.org

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