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Purified anti-mouse CD152 Antibody
产品名称:
Purified anti-mouse CD152 Antibody
产品类别:
抗体
产品编号:
106202
产品应用:
106202
[价格]
规格 价格 库存
500µg ¥ 1692 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
Mouse CTLA-4-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA - Quality tested
FC,?Block, Costim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No, 405204)). The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 106204).

Application References

(PubMed link indicates BioLegend citation)
  1. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block Costim FC)
  2. Krummel MF, et al. 1996. Int. Immunol. 8:519. (Block)
  3. Leach DR, et al. 1996. Science 271:1734. (Block)
  4. Wagner Jr, DH, et al. 1996. J. Exp. Med. 184:1631. (Block)
Product Citations
  1. Imazeki H, et al. 2021. J Immunother Cancer. 9:. PubMed
  2. Watanabe N, et al. 2016. PLoS One. 11: 0150244. PubMed
  3. Jang SC, et al. 2021. Commun Biol. 4:497. PubMed
  4. Wang S, et al. 2019. Cell Death Dis. 10:15. PubMed
RRID
AB_313247 (BioLegend Cat. No. 106202)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T cells

Function
Negative regulator of T cell activation
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.

Gene ID
12477 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

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