FITC anti-human CD49d Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1488	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Cat, Cow, Chimpanzee, Common Marmoset, Dog, Horse, Sheep, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation^2,3. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays?(Cat. No. 304339 and 304340).

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim)
Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed
Palacious F, et al. 2010. Blood 115:4488. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
Mattapallil MJ, et al. 2011. J. Immunol. 187:1977. PubMed

Product Citations

Buffone A, et al. 2018. J Cell Sci. 131:. PubMed
Horwacik I, et al. 2017. Int J Oncol. 50:1899. PubMed
Guo L, et al. 2019. Breast Cancer Res Treat. 175:459. PubMed
Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
Lee CH, et al. 2018. Elife. 7:e32532. PubMed

RRID

AB_2561758 (BioLegend Cat. No. 304315) AB_2561759 (BioLegend Cat. No. 304316)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, 150 kD.
Distribution: T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Function: Lymphocyte migration, T cell activation, stem cell differentiation.
Ligand/Receptor: Fibronectin, VCAM-1, MAdCAM-1
Cell Type: B cells, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4).
2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.
Gene ID: 3676 View all products for this Gene ID
UniProt: View information about CD49d on UniProt.org

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