Brilliant Violet 421™ anti-mouse CD16.2 (Fc&
- 产品名称：
- Brilliant Violet 421™ anti-mouse CD16.2 (Fc&
- 产品类别：
- 抗体
- 产品编号：
- 149521
- 产品应用：
- 149521
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- FCγR4 —EC domain fusion with IgG1 Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF? or Ultra-LEAF? purified antibody (Endotoxin < EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (contact our custom solutions team).
- Application References
(PubMed link indicates BioLegend citation) -
- Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block)
- Nimmerjahn F, et al. 2005. Immunity 23:41.
- RRID
- AB_2632892 (BioLegend Cat. No. 149521)
- Structure
- 22.4 kD
- Distribution
-
Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine
- Function
- Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
- Ligand/Receptor
- IgG2a and IgG2b
- Cell Type
- B cells, Leukocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Mechetina LV, et al. 2002. Immunogenetics. 54:463-8.
- Nimmerjahn F, et al. 2005. Immunity. 23:41-51.
- Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
- Gene ID
- 246256 View all products for this Gene ID
- UniProt
- View information about CD16.2 on UniProt.org
