Alexa Fluor® 700 anti-mouse CD16.2 (FcγR
- 产品名称:
- Alexa Fluor® 700 anti-mouse CD16.2 (FcγR
- 产品类别:
- 抗体
- 产品编号:
- 149507
- 产品应用:
- 149507
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- FCγR4 —EC domain fusion with IgG1 Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF? or Ultra-LEAF? purified antibody (Endotoxin < EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (contact our custom solutions team).
- Application References
(PubMed link indicates BioLegend citation) -
- Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block)
- Nimmerjahn F, et al. 2005. Immunity 23:41.
- RRID
- AB_2632741 (BioLegend Cat. No. 149507)
- Structure
- 22.4 kD
- Distribution
-
Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine
- Function
- Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
- Ligand/Receptor
- IgG2a and IgG2b
- Cell Type
- B cells, Leukocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Mechetina LV, et al. 2002. Immunogenetics. 54:463-8.
- Nimmerjahn F, et al. 2005. Immunity. 23:41-51.
- Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
- Gene ID
- 246256 View all products for this Gene ID
- UniProt
- View information about CD16.2 on UniProt.org