Pacific Blue™ anti-human CD335 (NKp46) Antib
- 产品名称：
- Pacific Blue™ anti-human CD335 (NKp46) Antib
- 产品类别：
- 抗体
- 产品编号：
- 331911
- 产品应用：
- 331911
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NKp46-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or per 100 ?l whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone 9E2 has been shown to block NK activation through NKp46.6
- Application References
(PubMed link indicates BioLegend citation) -
- Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
- Kalberer CP, et al. 2003. Blood 102:127.
- Chen Y, et al. 2007. J. Immunol. 179:2766.
- Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
- Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
- Achdout H. et al. 2010. J. Virol. 84:3993.
- Product Citations
-
- Irina Bancos, Jon Hazeldine 2017. Eur J Endocrinol. 176(4):471-480. PubMed
- Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
- Bancos I, et al. 2017. Eur J Endocrinol. 10.1530/EJE-16-0969. PubMed
- Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
- Pembroke T, et al. 2014. Gut. 63:515. PubMed
- RRID
- AB_2149281 (BioLegend Cat. No. 331911) AB_2149280 (BioLegend Cat. No. 331912)
- Structure
- Type I membrane glycoprotein (46 kD)
- Distribution
-
Expressed on resting and activated NK cells
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.
- Gene ID
- 9437 View all products for this Gene ID
- UniProt
- View information about CD335 on UniProt.org
