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Purified anti-c-Myc Antibody (Previously Covance c
产品名称:
Purified anti-c-Myc Antibody (Previously Covance c
产品类别:
抗体
产品编号:
904401
产品应用:
904401
[价格]
规格 价格 库存
100µL ¥ 4272 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC, IHC-P, IP - Verified
Purification - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25?- 1.0??g/mL.?For immunocytochemistry, a concentration range of 1.25?- 5.0?μg/mL is recommended.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5?- 5.0??g/mL?is suggested. For immunoprecipitation, the suggested use of this reagent is 2.5??g/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 9E11 monoclonal antibody recognizes the sequence 408-420 AEEQKLISEEDL of human c-myc.

This clone recognizes a non-specific band in BL21 E. coli lysates

BioLegend offers two clones against c-Myc, 9E10 and 9E11. When both clones were tested in parallel for WB, ICC, and IP, each clone performed similarly, with 9E11 showing slightly better performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Halme A, et al. 2004. Cell. 116:405.
  2. Kolodziej P, Young R. 1991. Meth. Enzymol. 194:508.
  3. Locker AP, et al. 1989. Brit. J. Cancer. 60:669.
Product Citations
  1. Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed
  2. Yi B, et al. 2022. Oncogene. 41:268. PubMed
  3. Wu Y, et al. 2016. Nat Struct Mol Biol. 10.1038/nsmb.3284. PubMed
RRID
AB_2565039 (BioLegend Cat. No. 904401)

Antigen Details

Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

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