Ultra-LEAF™ Purified anti-c-Myc Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Amino acids 408-439, C-terminal region of human c-myc

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

WB - Quality tested
ELISA, ICC, IP, IHC-P, Purification - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References (PubMed link indicates BioLegend citation)

Rao H, et al. 2001. Nature. 410:955.
Peters CS, et al. 2001. J Biol Chem. 276:13718.
Munro S, Pelham HR. 1987. Cell. 48:899.
Evan GI, et al. 1985. Mol Cell Biol. 5:3610.

RRID

AB_2810685 (BioLegend Cat. No. 626807) AB_2810686 (BioLegend Cat. No. 626808)

Antigen Details

Structure: Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
Distribution: Nuclear protein
Function: Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
Interaction: MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
Modification: Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
Cell Type: Embryonic Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Antigen References: 1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID: 4609 View all products for this Gene ID
UniProt: View information about c-Myc on UniProt.org

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