Purified anti-c-Myc Antibody
- 产品名称：
- Purified anti-c-Myc Antibody
- 产品类别：
- 抗体
- 产品编号：
- 626801
- 产品应用：
- 626801
- Verified Reactivity
- Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Amino acids 408-439, C-terminal region of human c-myc
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP, ICC, IHC-P - Verified
Purification - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g per ml. For immunoprecipitation, the suggested use of this reagent is 2.5??g/test.?For immunocytochemistry, a concentration range of 1.0 - 2.5 μg/mL is recommended.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0?- 5.0??g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
- Application References
(PubMed link indicates BioLegend citation) -
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- RRID
- AB_2235686 (BioLegend Cat. No. 626801) AB_2148451 (BioLegend Cat. No. 626802)
- Structure
- Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
- Distribution
-
Nuclear protein
- Function
- Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
- Interaction
- MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
- Modification
- Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
- Antigen References
-
1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.
- Gene ID
- 4609 View all products for this Gene ID
- UniProt
- View information about c-Myc on UniProt.org
