Direct-Blot™ HRP anti-c-Myc Antibody
- 产品名称：
- Direct-Blot™ HRP anti-c-Myc Antibody
- 产品类别：
- 抗体
- 产品编号：
- 626804
- 产品应用：
- 626804
- Verified Reactivity
- Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Amino acids 408-439, C-terminal region of human c-myc
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
- Application Notes
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
- Application References
(PubMed link indicates BioLegend citation) -
- Rao H, et al. 2001. Nature. 410:955.
- Peters CS, et al. 2001. J Biol Chem. 276:13718.
- Munro S, Pelham HR. 1987. Cell. 48:899.
- Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
- Product Citations
-
- Calvo IA, et al. 2021. iScience. 24:103338. PubMed
- RRID
- AB_2783361 (BioLegend Cat. No. 626804) AB_2572009 (BioLegend Cat. No. 626803)
- Structure
- Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
- Distribution
-
Nuclear protein
- Function
- Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
- Interaction
- MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
- Modification
- Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
- Antigen References
-
1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.
- Gene ID
- 4609 View all products for this Gene ID
- UniProt
- View information about c-Myc on UniProt.org
