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Purified anti-CHOP Antibody
产品名称:
Purified anti-CHOP Antibody
产品类别:
抗体
产品编号:
948701
产品应用:
948701
[价格]
规格 价格 库存
25µg ¥ 1416 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human CHOP protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.5?- 1.0??g/mL.?For immunocytochemistry, a concentration 5.0?μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed to detect CHOP in human small intestine.

For ICC testing, we do not recommend methanol only as a fixation-permeabilization step due to poor CHOP staining. We recommend 4% PFA fixation with either Triton X-100 or methanol permeabilization.

RRID
AB_2904462 (BioLegend Cat. No. 948701) AB_2904462 (BioLegend Cat. No. 948702)

Antigen Details

Structure
CHOP is a 169 amino acid protein with a predicted molecular weight of 19 kD.
Distribution

Expressed in cells and tissue undergoing ER stress/Nucleus

Function
Transcription Factor
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Misfolding and Aggregation, Transcription Factors
Antigen References

1. Yamaguchi, et al. 2004. J. Biol. Chem. 279: 45495
2. Oyadomari, et al. 2004. Cell Death Differ. 11:381.
3. Puthalakath H, et al. 2007. Cell. 129: 1337.

Gene ID
1649 View all products for this Gene ID
UniProt
View information about CHOP on UniProt.org

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