| 规格 | 价格 | 库存 |
|---|---|---|
| 25µg | ¥ 1416 | 1 |
WB - Quality tested
ICC, ICFC - Verified
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.5?- 1.0??g/mL.?For immunocytochemistry, a concentration 5.0?μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
This clone failed to detect CHOP in human small intestine.
For ICC testing, we do not recommend methanol only as a fixation-permeabilization step due to poor CHOP staining. We recommend 4% PFA fixation with either Triton X-100 or methanol permeabilization.
Expressed in cells and tissue undergoing ER stress/Nucleus
1. Yamaguchi, et al. 2004. J. Biol. Chem. 279: 45495
2. Oyadomari, et al. 2004. Cell Death Differ. 11:381.
3. Puthalakath H, et al. 2007. Cell. 129: 1337.