PerCP/Cyanine5.5 anti-human CD326 (EpCAM) Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD326 (EpCAM) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 324213
- 产品应用：
- 324213
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- DU.4475 breast carcinoma
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the revelant formats) include: immunofluorescence,?immunohistochemistry3, and spatial biology (IBEX)4,5.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Lammers R, et al. 2002. Exp. Hematol. 30:537.
- Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
- Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
- Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
- Willingham S, et al. 2012. Proc Natl Acad Sci U S A. 109:6627. PubMed
- Gui J, et al. 2011. Int Immunol. 1.407638889. PubMed
- Wang J, et al. 2020. Cell. 183:1264. PubMed
- Cai X, et al. 2014. PLoS One. 9:108942. PubMed
- Costa A et al. 2018. Cancer cell. 33(3):463-479 . PubMed
- Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
- Lau KX, et al. 2020. Nat Commun. 11:2420. PubMed
- Uniken Venema WT, et al. 2019. Gastroenterology. 156:812. PubMed
- Fridriksdottir A, et al. 2015. Nat Commun. 6: 8786. PubMed
- Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
- Kim J, et al. 2018. J Histochem Cytochem. 66:879. PubMed
- RRID
- AB_893474 (BioLegend Cat. No. 324213) AB_2098808 (BioLegend Cat. No. 324214)
- Structure
- Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
- Distribution
-
Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
- Function
- Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
- Modification
- Glycosylated.
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.
- Gene ID
- 4072 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
