- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- DU.4475 breast carcinoma
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB -?Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the revelant formats) include: immunofluorescence,?immunohistochemistry3, and spatial biology (IBEX)4,5.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
(PubMed link indicates BioLegend citation) -
- Lammers R, et al. 2002. Exp. Hematol. 30:537.
- Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
- Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
- Carraro G, et al. 2021. Nat Med. 27:806. PubMed
- Yu L, et al. 2020. Cell Stem Cell. 28(3):550-567.e12. PubMed
- Howden SE, et al. 2020. Cell Stem Cell. 28(4):671-684.e6. PubMed
- Palkowitz AL, et al. 2021. Adv Healthc Mater. 10:e2100132. PubMed
- Pal D et al. 2017. eLife. 6 pii: e21615. PubMed
- Pyo DH, et al. 2020. Cancer Biol Ther. 495:21. PubMed
- Morsing M, et al. 2020. Breast Cancer Res. 0.9875. PubMed
- Inde Z, et al. 2021. Sci Adv. 7:. PubMed
- Sullivan NT, et al. 2022. STAR Protoc. 3:101367. PubMed
- Cai X, et al. 2014. PLoS One. 9:108942. PubMed
- Calandrini C, et al. 2020. Nat Commun. 11:1310. PubMed
- Gerdur ísberg ó, et al. 2019. Sci Rep. 9:14843. PubMed
- Safaric Tepes P, et al. 2021. Elife. 10: . PubMed
- Hu H et al. 2018. Cell. 175(6):1591-1606 . PubMed
- Chen D, et al. 2019. Cell Rep. 29:4568. PubMed
- Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
- Gell JJ, et al. 2018. Stem Cell Res. 27:46. PubMed
- Hancock GV, et al. 2021. Stem Cell Res. 55:102493. PubMed
- Noort V, et al. 2014. Cancer Res. 74:5690. PubMed
- Morris K, et al. 2014. PLoS One. 9:98656. PubMed
- Hong HK, et al. 2019. Oncol Rep. 42:2029. PubMed
- Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed
- Vanslambrouck JM, et al. 2019. J Am Soc Nephrol. 30:1811. PubMed
- Staudte S, et al. 2022. Cancers (Basel). 14:. PubMed
- Vanslambrouck JM, et al. 2022. Nat Commun. 13:5943. PubMed
- Chen D, et al. 2017. Biol Reprod. 97:850. PubMed
- Cai X, et al. 2017. Neoplasia. 10.1016/j.neo.2017.03.005. PubMed
- Chitiashvili T, et al. 2020. Nat Cell Biol. 22:1436. PubMed
- Sandlin CW, et al. 2022. PLoS One. 17:e0274091. PubMed
- Pandolfi EC, et al. 2022. Cell Rep Med. 3:100782. PubMed
- Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
- Horwitz K, et al. 2008. Proc Natl Acad Sci U S A. 105:5774. PubMed
- Jeong HO, et al. 2022. iScience. 25:105358. PubMed
- Mourcin F, et al. 2021. Immunity. . PubMed
- Catenacci D, et al. 2015. Gastroenterology. 149: 1794-1803.e4. PubMed
- Forbes TA, et al. 2018. Am J Hum Genet. 102:816. PubMed
- RRID
- AB_756083 (BioLegend Cat. No. 324209) AB_756084 (BioLegend Cat. No. 324210)
