Purified anti-Nicastrin Antibody
- 产品名称：
- Purified anti-Nicastrin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 852301
- 产品应用：
- 852301
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to the C-terminal 18 a.a. of mouse Nicastrin.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 ?g per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
In human, the antibody also detects the deglycosylated nicastrin, which is observed at ~ 78kD.
- Application References
(PubMed link indicates BioLegend citation) -
- Esselens C, et al. 2004. J. Cell Biol. 166(7):1041. (WB)
- RRID
- AB_2728591 (BioLegend Cat. No. 852301) AB_2728592 (BioLegend Cat. No. 852302)
- Structure
- Deglycosylated nicastrin is a 709 amino acid protein with a molecular mass of ~78 kD. Higher molecular weight band of ~110 kD corresponding to glycosylated nicastrin is observed by WB.
- Distribution
-
Tissue Distribution: Ubiquitously expressed.
Cellular: Distribution: Plasma Membrane, endoplasmic reticulum, lysosome, golgi apparatus, and cytoskeleton.
- Function
- Nicastrin is an essential component of the γ-secretase complex that processes amyloid precursor protein to amyloid beta. It is also involved in degradation of amyloid beta fragments.
- Interaction
- Presenilin, APH-1, PEN-2
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
- Molecular Family
- Enzymes and Regulators, Secretases
- Antigen References
-
- Yang D, et al. 2002. J. Biol. Chem. 31:28135.
- Johnson DS, et al. 2017. Cold Spring Harb Perspect Med. 10.1101/cshperspect.a024067.
- Zhang X, et al. 2014. Front Cell Neurosci. 10.3389/fncel.2014.00427.
- Gene ID
- 23385 View all products for this Gene ID
- UniProt
- View information about Nicastrin on UniProt.org
