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Purified anti-mouse CD49d Antibody
产品名称:
Purified anti-mouse CD49d Antibody
产品类别:
抗体
产品编号:
103701
产品应用:
103701
[价格]
规格 价格 库存
50µg ¥ 996 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
(C57BL/6 x A/J)F1 mouse fetal liver mas
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3 of acetone-fixed frozen sections. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103709 & 103710).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
  3. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  4. Jia W, et al. 2005. Blood 106:3854. (FC)
Product Citations
  1. Jia W, et al. 2005. Blood. 106:3854. PubMed
  2. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  3. Lelouard H, et al. 2012. Gastroenterology. 142:592. PubMed
  4. Zhao D, et al. 2021. Cancers (Basel). 13: . PubMed
RRID
AB_313042 (BioLegend Cat. No. 103701)

Antigen Details

Structure
Integrin family, associates with integrin β1 or β7, 150 kD
Distribution

T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells

Function
T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor
VCAM-1, MAdCAM-1, fibronectin
Cell Type
B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.

Gene ID
16401 View all products for this Gene ID
UniProt
View information about CD49d on UniProt.org

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