PE anti-mouse CD49d Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

(C57BL/6 x A/J)F₁ mouse fetal liver mas

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation^1,2, and immunohistochemistry³ of acetone-fixed frozen sections. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103709 & 103710).

Application References (PubMed link indicates BioLegend citation)

Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Jia W, et al. 2005. Blood 106:3854. (FC)

Product Citations

Valanparambil RM, et al. 2017. PLoS Pathog. 13:e1006647. PubMed
Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
Di C, et al. 2015. J Biol Chem. 290:12523. PubMed
Simonetti G, et al. 2013. J Exp Med. 210:2887. PubMed
Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
Tran NL, et al. 2022. Elife. 11:. PubMed
Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed

RRID

AB_313046 (BioLegend Cat. No. 103705) AB_313047 (BioLegend Cat. No. 103706)

Antigen Details

Structure: Integrin family, associates with integrin β₁ or β₇, 150 kD
Distribution: T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function: T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor: VCAM-1, MAdCAM-1, fibronectin
Cell Type: B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
Gene ID: 16401 View all products for this Gene ID
UniProt: View information about CD49d on UniProt.org

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