Direct-Blot™ HRP anti-Insulin Degrading Enzy-上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 1632	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This antibody was raised against purified human erythrocyte IDE

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5?- 1.0??g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 9B12.225 has been shown to detect a ~118 kD isoform in peripheral tissue and a second isoform at ~54 kD in brain tissue.

Application References (PubMed link indicates BioLegend citation)

RG Bennett, et al. 2000. Endocrinology. 141:2508. (WB)
Chou YH, et al. 2009. FASEB J. 23:3734. (ICC) PubMed

RRID

AB_2861113 (BioLegend Cat. No. 921205) AB_2861114 (BioLegend Cat. No. 921206)

Antigen Details

Structure

IDE is a 1019 amino acid protein with a molecular weight of ~ 118 kD.

Distribution

Tissue distribution: Abundantly expressed in gastrointestinal tract, muscle and nervous system tissues.
Cellular distribution: Secreted, mitochondrion, peroxisome, nucleus, cytosol, and plasm membrane.

Function

IDE cleaves the peptide hormone insulin with consequences for insulin response and resistance. IDE is also the enzyme responsible for degradation and clearance of amyloid beta in the brain.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance

Molecular Family

Enzymes and Regulators, Proteases

Antigen References