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首页 > 抗体蛋白 > 抗体
Purified anti-mouse CD16/32 Antibody
产品名称：
Purified anti-mouse CD16/32 Antibody
产品类别：
抗体
产品编号：
101301
产品应用：
101301
[价格]
规格 价格 库存
50µg ￥ 432 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 ?g per 106 cells in 100 ?L volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered).

Application References

(PubMed link indicates BioLegend citation)
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RRID
AB_312800 (BioLegend Cat. No. 101301) AB_312801 (BioLegend Cat. No. 101302)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

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